AdvanceBio Oligonucleotide Columns with new sustainable packaging
AdvanceBio Oligonucleotide Columns
AdvanceBio Oligonucleotide columns offer high-quality, high-resolution ion-pair reversed-phase (IP-RP) column chemistry platform that support analytical characterization through purification with seamless method scale-up. To successfully separate trityl-off, deprotected oligonucleotides, HPLC columns need to have high resolving power and be robust enough to withstand high pH and temperature, which provide better separation and denaturing conditions for optimal resolution and oligonucleotide separation.
AdvanceBio Oligonucleotide columns feature high-efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio Oligonucleotide media with a resolution standard to ensure consistent performance. AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities, and biopharmaceuticals.
AdvanceBio Oligonucleotide columns feature high-efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio Oligonucleotide media with a resolution standard to ensure consistent performance. AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities, and biopharmaceuticals.
- Oligonucleotide Analysis Columns and Standards
This product is not available for purchase by the general public.
Product Details
Features
- Robust particle chemistry with high pH and temperature stability – allows for increased resolution under optimal denaturing conditions for superior peak shape and separation
- Fully scalable particle chemistry platform available in 2.7 and 4 μm Poroshell particle – simplifies method scale-up and eliminates need for chemistry re-validation when scaling up to purification
- 4 μm Poroshell particle chemistry in 21.2 mm ID preparative columns – higher efficiency and increased resolution for improved yield and purity of target product
- Increased flexibility – compatible with HPLC and UHPLC instruments via 2.7 μm Poroshell particles
- Improved reliability of results – high-resolution separations delivered by efficient Poroshell particle morphology
- Reduced costs – long column lifetimes from robust, high-pH resistant, chemically modified silica
Tools
Bio HPLC Columns eSelector Tools
Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.Oligonucleotide Virtual Lab
Explore proven methods and resources to help advance analysis for the broad range of oligonucleotide workflows.Preparative HPLC Scaling Calculator
Scale between analytical and prep methods; calculate column capacity, # of injections, run time & mobile phase use.How It Works
Videos
Literature
- Key Literature
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Superficially Porous Columns for Semi-Preparative Purification of Synthetic Oligonucleotides
AdvanceBio superficially porous columns for excellent performance and resolution for semi-prep purification of synthetic oligos without compromising column loading.- Application Notes
- English
- 03 Jun 2024
- 683.32 KB
Molecular Weight Confirmation of Oligonucleotides Using Agilent LC/MSD XT and OpenLab CDS
Molecular weight confirmation and simple characterization workflows for synthetic oligos with Agilent LC/MSD XT and MS Spectral Deconvolution feature in OpenLab CDS 2.8.- Application Notes
- English
- 29 Feb 2024
- 410.29 KB
Maximize Your Resources and Simplify Scale-up with AdvanceBio Oligonucleotide Columns
Competitive comparison from analytical to semipreparative purification of synthetic oligonucleotides.- White Papers
- English
- 29 Jul 2024
- 877.84 KB
- Application Notes
- Brochures
- Catalogs
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Support
- FAQs
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Bio LC Column User Guides
User guides contain helpful information to ensure the best performance from your column, such as operating parameters, cleaning and storage guidelines, usage tips, and suggested starting conditions. - User Manuals
Quick Links to Oligonucleotide Portfolio
Related Products
News
Semi-Preparative Purification of Synthetic Oligonucleotides
This application note discusses the optimum conditions for purification of a synthetic oligonucleotide and scaling up to a 10 mm internal diameter column using the same analytical HPLC instrument. The latest developments include columns packed with superficially porous particles that provide excellent performance and resolution without compromising column loading.- 03 Jun 2024
Agilent Launches New AdvanceBio Spin Columns For Desalting and Buffer Exchange
AdvanceBio Spin Columns address the need for efficient sample preparation of proteins or oligonucleotides under aqueous conditions, preserving native structure with suitable ionic strength and pH. Users can easily and quickly remove excess salts or undesired small molecules from their sample matrix or exchange the sample matrix between different buffer conditions. These gel-filtration-based spin columns are available in analytical and semipreparative scale columns for single samples, as well as 96-sample plates for high-throughput settings.- 01 Apr 2024
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